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Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

If you experience signs of an allergic reaction such as hives, difficulty breathing, or swelling of your face, lips, tongue, or throat, seek emergency medical attention immediately.

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COA Heat stress response refers to the total response of the body to any demands made by the heat environment when it is in a high temperature environment, characterized by an increase in the synthesis of heat shock proteins (HSPs)