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Cells were treated for 24 h after seeding and exposed for 72 h (Figure 1)

3.3 MAPKAPK2 overexpression reduces ROS accumulation and restores antioxidant capacity in H 2 O 2 -treated oxidative stress To evaluate the antioxidant regulatory function of MAPKAPK2, we assessed intracellular ROS levels and key antioxidant markers in H 2 O 2 -treated PIG3V cells with or without MAPKAPK2 overexpression

Chronic consumption may alter gastrointestinal acidity, nutrient bioavailability, and renal function, highlighting the need for more research to establish appropriate safety guidance