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RPMI/B27 medium (61,870,036), B27 supplement (17,504,044), GlutaMax supplement (35,050,061), Insulin- Transferrin-Selenium supplement (41,400,045), Knock Out DMEM (10,829,018), KnockOut Serum Replacement (10,828,028), Fetal Bovine Serum (A3840002), MEM Non-Essential Amino Acids Solution (11,140,050), Geltrex (A1413302), Dulbeccos phosphate-buffered saline, calcium and magnesium free DPBS / (14,190,144), ProLong Gold Antifade Mountant with DAPI (P36935), Trypsin (90,057), Trizol Reagent (15,596,026) were from Thermo Fisher Scientific (Rockford, IL)

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The number of macroscopic colonies in the spleen was counted after 12 days, following fixation in Bouins solution [2]

That gap widens over time, from roughly 4-5 percentage points at 24 weeks to over 6 percentage points by 68-72 weeks