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Blocking and permeabilization were performed for 1 h at room temperature in PBS containing 2% BSA and 0.1%, 0.2%, 0.5% or 1% Triton X-100 depending on the antigen (Supplementary Data 5)

Mitochondrial dysfunction in Kupffer cells plays a central role: IR causes Kupffer cell mitochondria to release mtDNA and ROS, activating NLRP3 inflammasomes which then release IL-1 that amplifies neutrophil recruitment (67)

PMID: 161 TurrellFKOrhaRGuppyNJGillespieAGuelbertMStarlingCet al

All samples were acquired on BD LSRFortessa (BD Biosciences)

However, the reduction of the intermolecular disulfide of Prx is specific to Trx and cannot be achieved by GSH or Grx (Chang et al., 2004