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To further demonstrate covalent modification of this scaffold with GCLM, we synthesized an alkyne-functionalized analog of EN25 with EN25-alkyne that demonstrated an IC 50 for GCL activity of 3.6 M

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The untreated and MSO-treated parasite pellets isolated by saponin treatment were pooled separately during solubilization with 6 M urea in 25 mM ammonium bicarbonate buffer

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