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While BTK inhibitors have shown efficacy in subsets of ABC-DLBCL [38], their activity as single agents is frequently limited by the emergence of adaptive resistance and incomplete suppression of tumor survival programs

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Finally, data analysis was performed using the FlowJo software for quantifying the percentage of positive cells (uptake [%]) as well as the relative mean fluorescence intensity per cell (rMFI) according to: Evaluation of Transfection Efficiency via Flow Cytometry For calculating the siRNA gene silencing efficiency on eGFP-H1299 cells, 7,500 cells per well were seeded (for 48 h study) in 96-well plates which were allowed to attach overnight