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Results Gpx3 is upregulated in BALF and tissue in the mouse model of bleomycin-induced pulmonary fibrosis Initially, in order to assess expression and regulation of lung-abundant extracellular antioxidant proteins, we extracted the relevant data from a previously published proteomic study on bleomycin-induced pulmonary fibrosis 35

We also observed that 90% of the enzyme activity was lost when reactions were conducted in a DTT-containing (100 mM) reaction solution, likely because glycine oxidation cannot proceed and/or C281 is fully reduced (underscoring the essentiality of the post-translational modification) (Supplementary Table 3)