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Briefly, L-lysine (0.78 g, 5 mmol) and D, L-Hcy thiolactone hydrochloride (0.93 g, 5 mmol) were dissolved in 100 mL 0.2 M sodium phosphate buffer, pH 7.4, 0.2 mM EDTA and allowed to react for 24 h at room temperature

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The effects last for 8 days to 3 weeks depending on the condition, cocktail and type of IV Therapy