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Quantitative accuracy is enhanced through incorporation of stable isotope-labeled synthetic peptides as internal standards (Liquid Chromatography-Stable Isotope Dilution-MRM MS or LC-SID-MRM MS), a methodology successfully applied for absolute quantification of CFTR protein using signature peptides as full-length protein surrogates (McShane et al., 2014)

doi: 10.1093/cvr/cvad023 182 ZhouX.JiS.ChenL.LiuX.DengY.YouY.et al

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Four Polar bodies and two cumulus cell samples were also analyzed to serve as a control for genome amplification and Sanger sequencing

Int J Mol Sci Aug