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Following separation, desired species-containing fractions were pooled and concentrated by ultrafiltration (Millipore Amicon Ultra-15/4 centrifugal filters

The selenocysteine bearing subunits of the enzymes catalytic site are viewed as cysteine analogues and are vulnerable to electrophilic attack by compounds with conjugated carbonyl systems

SAM acts primarily as a universal methyl donor in the synthesis of methylated compounds such as neurotransmitters (adrenaline, noradrenaline), DNA, RNA, phosphatidylcholine and creatine ( Reference Brosnan, da Silva and Brosnan 14 )

Dizziness or lightheadedness may occur in some patients after the injection, possibly related to an autonomic nervous system response or mild hypotension following the dose