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Cells were then spun at 600 g for 3 min, washed with PBS, and resuspended in Triton X-114 lysis buffer (20 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-114) containing freshly added protease and phosphatase inhibitors including leupeptin (1 g/ml), aprotinin (1 g/ml), pepstatin (1 g/ml) and PMSF (200 M)

[DOI] [PMC free article] [PubMed] [Google Scholar] 281.Rose WC

FAQ Some patients begin noticing reduced pain, improved mobility, and accelerated recovery within the first few weeks

To investigate the possible pathway of the preferential uptake of 2 NP over 1 NF , we evaluated NP internalization under conditions that suppressed cellular energy production

Konoplev S, Lin P, Yin CC, Lin E, Nogueras Gonzalez GM, Kantarjian HM, Andreeff M , Medeiros LJ, Konopleva M