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Based on spectroscopic studies it was suggested that His forms a ternary complex with CuHSA 34 , however, a direct [ 14 C]His binding assay by using ultrafiltration showed that His interaction with CuHSA could be only transient 8

The model achieved area under the receiver operating characteristic curve values of 0.76 for oligospermia, 0.89 for asthenozoospermia, and 0.86 for teratozoospermia, indicating its potential as a non-invasive diagnostic tool (172)

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