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DOI: 10.1529/biophysj.107.112367

The assays were performed with the supernatants as described for the recombinant proteins with the inclusion of 200 M phosphoenolpyruvate (Sigma-Aldrich, P0564) and 6 Units of pyruvate kinase (Sigma-Aldrich, P7768) to ensure the regeneration of ATP in the parasite lysates 71

Thus, retrograde transport mainly mediates the transport of VSRs from the TGN to the Golgi apparatus and/or the ER to engage in subsequent rounds of cargo protein sorting 22,23