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Total RNA extraction and quantitative reverse transcription polymerase Chain reaction (qRT-PCR) The effect of APE on pro-inflammatory cytokine gene expression from LPS-stimulated RAW264.7 cells was determined using a qRT-PCR assay

The blots were incubated with a primary antibody against GLO1 (1:200, ABCAM, United States) and -actin (1:2,000, Cell Signaling Technology, United States) overnight at 4C according to the instructions of the manufacturer

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The selection of the 20 pectoralis major muscles was based on the interquartile range that captures 50% of the data points in the aforementioned dataset