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During mass spectrometry data acquisition, electrospray ionization was used with the operational parameters included a source probe temperature set at 450 C, a voltage of either 4,500 V or -4,500 V, a curtain gas maintained at 30 psi, an ion source gas pressure of 30 psi, and a declustering potential applied at either 100 V or -100 V

Initial Treatment Dosage: 1000 mcg Daily Protocol Adults with a severe B12 deficiency usually start with 1000 mcg daily for one week

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Furthermore, specific bacterial and prebiotic interventions are generally well tolerated, easily standardized and repeatable, and present a lower practical and ethical barrier compared to FMT