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The resin was washed once with His lysis buffer and three times with His 6 wash buffer (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 20 mM imidazole, 5% glycerol, 2.5 mM ME)

The trade-off is a lower per-capsule dose (Thornes is 175 mg per capsule, compared to 500 mg in standard capsules)

Vitamin D is naturally present in very few foods (i.e., fatty fish and fish oil, with smaller quantities in mushrooms, egg yolks, and liver) but is commonly added to cereals and bread products

In addition, heterogeneity between the studies that reported data on UPDRS was low ( 2 =7.51, I 2 =20%, P=0.28) and thus, the fixed-effects model was applied once again (Fig