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Similarly, microglial activation with lipopolysaccharide and interferon- has been proposed to activate a microglia-astrocyte-neuron lactate shuttle, particularly in response to excitotoxic stimuli ( FIGURE 2 Astrocyte-neuron metabolic coupling established mostly through MCTs is the underlying molecular mechanism for the astrocyte-neuron lactate shuttle and brain energetic support (Pellerin et al., 1998a

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In contrast, the label-based method uses a secondary antibody conjugated with enzymes, metal nanoparticles, or redox mediators, amplifying the current generated after antigen binding [158]

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