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Sybody selectionsmall-scale pull-down assay to identify TGP binders To screen TGP binders at a single-colony level, sybodies expressed in the periplasmic space were extracted first as follows

Observe the behavior, weight changes and death of the animals to determine the maximum tolerated dose (MTD) or median lethal dose (LD50)

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Although the use of primary brain endothelial cells and/or co-culture with other cells would provide a physiologically more relevant environment, bEND.3 cells consistently show a stable barrier function and thus have been widely used in previous studies on BBB permeability to therapeutics 35

Several randomized, placebo-controlled trials indicate that metformin therapy leads to a progressive decline in vitamin B12 levels and can cause physiologically significant deficiency (153)