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1 H-NMR(D 2 O, ppm): 2.22.3(m, 2H, CH 2 CO 2 NBu 4 ), 2.52.8(m, 2H), 3.23.3(m, 1H, 3-H) 13 C-NMR(D 2 O, ppm): 41.09(CH 2 CO 2 NBu 4 ), 48.23(4-CH 2 ), 51.14(3-CH), 178.54(CO 2 NBu 4 ) Triethylamine (790 mg, 7.81 mmol) was added to the reacting solution of D 2 O containing (S)-4-hydroxy-3-methanesulfonyl hydroxybutyric acid, so prepared from Example 3 and stirred at room temperature for 30 minutes

To label nuclei, hair follicles were incubated for 30 to 45 min with Hoechst 33258 (life Technologies, stock at 10 mg/mL) diluted 2000x in supplemented Williams medium

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As expected, at the end of the experiment, HFD mice gained 20% more BW than STD mice (Supplementary Figure S1A), together with increased heart weight (HW) and serum NEFA (Supplementary Figures S1BD)

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