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Studies show the peptide increases dendritic spine density by 40-60% in treated brain regions, with formation of new functional synaptic connections confirmed by electrophysiological recordings demonstrating enhanced synaptic transmission and long-term potentiation

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After fixation, the cells were washed with PBS and permeabilized with 0.1% Triton X-100 (Daejung 8566-4405) in PBS for 10 min at room temperature

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