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Oxidative stress, marked by an excess of ROS relative to antioxidant systems, is a key pathological mechanism in hypertension

J Proteome Res 7:31373145 Soga T, Baran R, Suematsu M, Ueno Y, Ikeda S, Sakurakawa T, Kakazu Y, Ishikawa T, Robert M, Nishioka T, Tomita M (2006) Differential metabolomics reveals ophthalmic acid as an oxidative stress biomarker indicating hepatic glutathione consumption

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Briefly, cells were seeded in the 6-well plate with 3 10 5 cells per well and transfected with 2 g plasmids using 6 L Lipofectamine 2000 regent